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KMID : 0387720100210030230
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Korean Journal of Blood Transfusion
2010 Volume.21 No. 3 p.230 ~ p.235
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Characteristics of Intron 6 Nucleotide Sequences of cis-AB in Koreans
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Kim Ha-Nui
Lee Hye-Jin
Choi Gye-Ryung
Kim Myung-Han
Kim Jang-Su
Lim Chae-Seung
Kim Young-Kee
Lee Kap-No
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Abstract
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Backgound: The cis-AB is a very rare phenotype in the ABO blood group system. It corresponds to a special ABO allele that encodes glycosyltransferase that is capable of synthesizing both A and B antigens. Until now, the exon 6 and 7 gene sequences of cis-AB alleles are well known. In this study, we report on the intron 6 sequence structure of the cis-AB allele.
Methods: Standard serologic tests for the ABO blood group phenotypes were performed in four cis-AB samples. Allele-separation by cloning and subsequent sequencing was carried out.
Results: The results showed that intron 6 of cis-AB is almost identical to the A101 allele except for three single nucleotide polymorphisms at nucleotide positions 163, 179 and 662, where the nucleotides of the A101 replace those of B101.
Conclusion: The intron 6 sequences of cis-AB in Koreans have both A101 and B101 blood group sequences.
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KEYWORD
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cis-AB, Blood typing, ABO blood group, Glycosyltransferase, Intron 6
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